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KMID : 0604520060320030161
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Journal of the Society of Cosmetic Scientists of Korea
2006 Volume.32 No. 3 p.161 ~ p.171
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Preliminary Screening of Some Jeju Island Native Plants for Whitening and Antioxidant Activity
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Yoo Byung-Sam
Moon Ji-Young
Kim Joo-Ho
Hyun Jin-Won
Kang Kyung-Ah
Koh Jae-Sook
Seo Young-Kyung
Baek Ji-Hwoon
Park Deok-Hoon
Lee Jong-Sung
Jung Eun-Sun
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Abstract
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In this study, we investigated the whitening and anti-oxidant activity of 37 Jeju island native plants. The active ingredients of the plants were prepared by methanol extraction. Whitening activity of plant extracts was examined from the inhibitory effect of tyrosinase and the inhibition of melanin synthesis of the B16-F1 cell line. Their anti-oxidant activity was measured by electron donating ability of DPPH (1,1-diphenyl-2-picrylhydrazyl) and ROS (reactive oxygen species) scavenging activity in V79-4 lung fibroblast cells using DCF-DA (dichlorofluorescin diacetate). Cytotoxicity of the extracts on cell s based experiments was investigated by MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay. Also, the toxicity test using a rabbit and the human skin patch test were carried out for examining the safety of the extracts which showed the high whitening activity. It is interesting that the extracts of Lespedeza cuneata, Ligustrum lucidum (stem), Morus bombycis (stem) and Prunella vulgaris var. lilacina showed both potent whitening and anti-oxidant activities.
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KEYWORD
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tyrosinase, DPPH, DCF-DA, MTT, skin-patch test
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